Plasmid

Part:BBa_K3027003:Design

Designed by: Arnaud Boudigou   Group: iGEM19_GO_Paris-Saclay   (2019-09-24)


pBAD24-nuclease_A1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1311
    Illegal PstI site found at 3018
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1311
    Illegal NheI site found at 1300
    Illegal PstI site found at 3018
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1311
    Illegal BamHI site found at 1239
    Illegal BamHI site found at 1331
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1311
    Illegal PstI site found at 3018
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1311
    Illegal PstI site found at 3018
    Illegal NgoMIV site found at 4581
    Illegal NgoMIV site found at 6159
    Illegal AgeI site found at 1074
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3202
    Illegal BsaI site found at 4269
    Illegal SapI site found at 1056
    Illegal SapI.rc site found at 5788


Design Notes

The A1 nuclease CDS has been inserted between the two Bsa1 sites


Source

TBD

References